Application Note | Fossil Surface Analysis
Fossil Microstructure Analysis Using a Portable 3D Profilometer
Non-Contact 3D Measurement of Brachiopod and Crinoid Stem Fossil Surfaces for Paleontology and Geology

Research & Experimental Testing
Duanjie Li
Visual Design & Editorial
Andrew Shore
Introduction
Fossils are the preserved remains of traces of plants, animals and other organisms buried in sediment under ancient seas, lakes and rivers. The soft body tissue usually decays after death, but the hard shells, bones and teeth fossilize. Microstructure surface features are often preserved when mineral replacement of the original shells and bones takes place, which provides an insight into the evolution of weather and the formation mechanism of fossils.
Why Use a 3D Non-Contact Profilometer for Fossil Examination
3D profiles of the fossil enable us to observe the detailed surface features of the fossil sample from a closer angle. The high resolution and accuracy of the NANOVEA profilometer reveal details that may not be discernible by the naked eye. The profilometer’s analysis software offers a wide range of studies applicable to these unique surfaces. Unlike other techniques such as touch probes, the NANOVEA 3D Non-Contact Profilometer measures the surface features without touching the sample. This allows for the preservation of the true surface features of certain delicate fossil samples. Moreover, the portable model Jr25 profilometer enables 3D measurement on fossil sites, which substantially facilitates fossil analysis and protection after excavation.
ℹ️ Learn more about non-contact profilometry lab services for geological and natural samples.
Measurement Objective
In this study, the NANOVEA Jr25 Profilometer is used to measure the surface of two representative fossil samples. The entire surface of each fossil was scanned and analyzed in order to characterize its surface features which include roughness, contour and texture direction.
Brachiopod Fossil
The first fossil sample presented in this report is a Brachiopod fossil, which came from a marine animal that has hard “valves” (shells) on its upper and lower surfaces. They first appeared in the Cambrian period, which is more than 550 million years ago.
The 3D View of the scan is shown in Fig. 1 and False Color View is shown in Fig. 2.


Fig. 1: 3D View of the Brachiopod fossil sample.

The overall form was then removed from the surface in order to investigate the local surface morphology and contour of the Brachiopod fossil as shown in Fig. 3. A peculiar divergent groove texture can now be observed on the Brachiopod fossil sample.

A line profile is extracted from the textured area to show a cross-sectional view of the fossil surface in Fig. 4. The Step Height study measures precise dimensions of the surface features. The grooves possess an average width of ~0.38 mm and depth of ~0.25 mm.

| Step | Width (mm) | Max depth (mm) | Mean depth (mm) |
|---|---|---|---|
| Step 1 | 0.391 | 0.354 | 0.297 |
| Step 2 | 0.532 | 0.276 | 0.243 |
| Step 3 | 0.258 | 0.221 | 0.204 |
| Step 4 | 0.274 | 0.227 | 0.208 |
| Step 5 | 0.336 | 0.301 | 0.276 |
| Step 6 | 0.477 | 0.312 | 0.277 |
| Step 7 | 0.391 | 0.259 | 0.205 |
Fig. 4: Line profile and Step Height studies of the textured surface.
Crinoid Stem Fossil
The second fossil sample is a Crinoid stem fossil. Crinoids first appeared in the seas of the Middle Cambrian Period, about 300 million years before dinosaurs.
The 3D View of the scan is shown in Fig. 5 and False Color View is shown in Fig. 6.


Fig. 5: 3D View of the Crinoid fossil sample.

The surface texture isotropy and roughness of the Crinoid stem fossil are analyzed in Fig. 7. This fossil has a preferential texture direction in the angle close to 90°, leading to texture isotropy of 69%.

| Texture parameter | Value |
|---|---|
| Isotropy | 69.5% |
| First direction | 87.2° |
| Second direction | 93.8° |
| Third direction | 81.7° |
| ISO 25178 height parameter | Value |
|---|---|
| Sa | 0.0648 mm |
| Sq | 0.124 mm |
| Ssk | −4.67 |
| Sku | 37.5 |
| Sp | 0.309 mm |
| Sv | 1.41 mm |
| Sz | 1.72 mm |
Fig. 7: Surface texture isotropy and roughness of the Crinoid stem fossil.
The 2D profile along the axial direction of the Crinoid stem fossil is shown in Fig. 8. The size of the peaks of the surface texture is fairly uniform.

| Step | Width (mm) | Max depth (µm) | Mean depth (µm) |
|---|---|---|---|
| Step 1 | 0.119 | 97.3 | 86.8 |
| Step 2 | 0.196 | 133 | 103 |
| Step 3 | 0.154 | 137 | 107 |
| Step 4 | 0.098 | 95.0 | 81.5 |
| Step 5 | 0.112 | 99.2 | 84.3 |
| Step 6 | 0.763 | 202 | 172 |
| Step 7 | 0.413 | 116 | 88.2 |
| Step 8 | 0.112 | 90.1 | 80.9 |
Fig. 8: 2D profile analysis of the Crinoid stem fossil.
Conclusion
In this application, we comprehensively studied the 3D surface features of a Brachiopod and Crinoid stem fossil using the NANOVEA Jr25 Portable Non-Contact Profilometer. We showcase that the instrument can precisely characterize the 3D morphology of the fossil samples. The interesting surface features and texture of the samples are then further analyzed. The Brachiopod sample possesses a divergent groove texture, while the Crinoid stem fossil shows preferential texture isotropy. The detailed and precise 3D surface scans prove to be ideal tools for palaeontologists and geologists to study the evolution of lives and the formation of fossils.
The data shown here represent only a portion of the calculations available in the analysis software. NANOVEA Profilometers measure virtually any surface in fields including Semiconductor, Microelectronics, Solar, Fiber Optics, Automotive, Aerospace, Metallurgy, Machining, Coatings, Pharmaceutical, Biomedical, Environmental and many others.
Frequently Asked Questions About 3D Fossil Surface Analysis
Why scan fossils with a 3D non-contact profilometer?
Unlike touch probes, a non-contact profilometer measures surface features without touching the sample, preserving the true surface of delicate fossils. Its high resolution and accuracy reveal details not discernible by the naked eye, and the analysis software offers a wide range of studies for these unique surfaces.
Can fossils be measured at the excavation site?
Yes. The portable NANOVEA Jr25 profilometer enables 3D measurement on fossil sites, which substantially facilitates fossil analysis and protection after excavation.
What surface features can be measured on a fossil?
Roughness, contour and texture direction. After form removal, the Brachiopod fossil revealed a divergent groove texture whose grooves average ~0.38 mm wide and ~0.25 mm deep in a Step Height study.
What does texture direction show on the Crinoid stem fossil?
The Crinoid stem fossil has a preferential texture direction close to 90°, with a first direction of 87.2° and a texture isotropy of 69.5%. A 2D profile along its axis shows fairly uniform peaks.
What roughness was measured on the Crinoid stem fossil?
ISO 25178 height parameters of Sa 0.0648 mm, Sq 0.124 mm, Sp 0.309 mm, Sv 1.41 mm and Sz 1.72 mm, with a skewness Ssk of −4.67 and a kurtosis Sku of 37.5.
Which NANOVEA instrument was used for fossil scanning?
The NANOVEA Jr25 Portable Non-Contact Profilometer. NANOVEA profilometers measure virtually any surface, in fields from semiconductors and coatings to biomedical and environmental research.



